Founded in 1666, Truman's used to brew its beer in Brick Lane in east London but production stopped in 1989.
James Morgan and Michael-George Hemus refounded the brand in 2010 and have been brewing under licence in Essex since then. Its flagship beer - Truman's Runner - is currently available in more than 150 pubs across London.
James Morgan, one of the founders of the New Truman's Brewery, said: "We are delighted to be bringing 346 years of brewing heritage back to the local community. This is the biggest brewing investment in East London in several decades. The site is in Hackney Wick, one of the most exciting areas in East London. You can see the Olympic Stadium from the front door"
Initial investment has been secured and the company's 'Truman's Eagle' funding scheme closes at the end of the year. The official opening of the New Truman's Brewery is planned for the spring.
Michael-George Hemus, who re-launched the new brewery alongside Morgan, added: "It all seemed a bit daunting when we set out to bring Truman's back to East London. But we have had great support from the East London community, with a number of local people investing directly in the project via our "Truman's Eagle" scheme. We want to make the Truman's name great again - and we are only just getting started."